Influence of Caffeine on Crystallization and Amelioration of Oxidative Stress on in vitro Model of Urolithiasis

Vjera Ninčević, Tomislav Kizivat, Tea Omanović Kolarić, Lucija Kuna, Anita Cindrić, Anamarija Banovac, Ines Bilić Ćurčić, Sandra Tucak-Zorić, Martina Smolić


Urolithiasis is a disease characterized by formation of solid crystals within the urinary tract. Kidney stone formation
is still not clear but it is mostly composed of calcium oxalate which can produce free radicals that are toxic to renal tubular
cells. Oxidative stress is an important contributory mechanism in cell damage and is associated with a number of
disorders. Several studies have shown antioxidative effects of caffeine, proposing its possible role in stopping the formation
of calcium oxalate stones in urinary tract. Hence, the aim of this study was to evaluate the toxic effects of calcium oxalate
monohydrate crystals (COM) on renal epithelial cell line; Madin-Darby canine kidney cells subtype I (MDCK I) and
Epithelial-like pig kidney cell line (LLC-PK1), and to determine possible inhibition of COM that caused oxidative stress
by antioxidant treatment with caffeine in different concentrations in a cell culture model of urolithiasis.


urolithiasis, oxidative stress, MDCK cells, LLC-PK1 cells, calcium oxalate monohydrate, caffeine

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